Welcome!
Cell Analysis and Sorting
The Core provides analysis and sorting of various cell types on two Becton Dickinson cell sorting instruments.
Cell analysis and cell sorting are done on either the BD FACSVantage sorter or the BD FACSAria IIu sorter.
Billing Policy
IDDRC Stem Cell (Flow Cytometry/ Cell Sorter) Core
The IDDRC Stem Cell (Flow Cytometry/ Cell Sorter) Core is a fee for service facility. Our Core Manager and Operators
want to be sure that all our Investigators understand our billing policy before booking time on the sorters.
We will provide assisted service to Investigators who bring cells to sort and/ or analyze as Assisted Users.
We will offer training for Investigators who want to learn how the FACS Aria IIu operates and how the software
functions to tailor experiments to acquire the desired cells. These Unassisted Users will be able to run the Quality Control
for the Drop 1 values, set up the graphs, gates and link the gates for their experiment. They will
set up the sort layout and side stream positions for collecting their sorted cells.
We will offer further training for Investigators who want the opportunity to independently run their experiments
whenever their samples are ready as Expert Users. The Expert Users will be able to start the FACS Aria, perform
the daily QC and set up the Laser Delay and daily Drop 1 values, set up their experiment, sort layout and sort positions
for collecting their cells and clean and shut down the FACS when they have finished their experiment.
Unassisted Users will need to use the FACS a minimum of 4 times in 4 months to retain their status as an Unassisted User
and Expert Users will need to use the FACS a minimum of 6 times in 4 months to retain their status as an Expert User.
Assisted Users receive priority for advanced reservations during weekday work hours. Unassisted Users may sign up for any
time during weekday work hours. Expert Users may sign up for any time that meets the needs of their research. In the rare
occasion that an Assisted User requests a weekday time slot that has been reserved by an Unassisted User or Expert User
and no other time slot will meet this Assisted User’s needs, the Manager or Operator may require that an Investigator who has
signed up for unassisted use either opts to pay at the assisted use rate or reschedule the appointment. At least 24 hour notice
will be given for rescheduling. You will be charged for the time you reserved in iLab, but the core manager may increase or
(in rare instances) decrease that time charged based on your actual use. The charges are rounded UP to the nearest 15 minutes.
There is a minimum charge of one hour. Assisted use is only available until 5:30 PM on weekdays. Reservations must be canceled
24 hours before the time scheduled.
Failure to cancel in time will result in charges for the full time reserved.
Unassisted users who require more than 7 or 8 minutes of assistance during an experiment will be charged at the assisted user rate.
Expert users who book experiments that end before 5:30 PM on weekdays will be charged at the unassisted rate unless, as above,
they require more than 7 or 8 minutes of assistance.
See Dana-Farber Policies for additional policy information.
Table of Hourly Charges for Services
|
|
Core Affiliate |
BCH |
Outside/Research |
Industry |
|
Assisted User |
$80.00 |
$90.00 |
$110.00 |
$130.00 |
|
Unassisted User |
$56.00 |
$63.00 |
$77.00 |
$91.00 |
|
Expert User |
$40.00 |
$45.00 |
$55.00 |
$65.00 |
|
Training |
$40.00 |
$45.00 |
$55.00 |
$65.00 |
How to request services:
Before you can login and request services, you must register an iLAB account by clicking on the "sign up here" link
at the top-right of the opening page of the website and fill in all the required information. Once registration is completed,
please login and click on Equipment Reservations tab. Select the instrument and view schedule, then YOU MUST click and DRAG
(not just click) on the time slot where you want to start your reservation.
How can I properly estimate how much time my sort or analysis will take?
In order not to overbook or underbook the amout of time you need to schedule so that your PI does not get charged
for time you booked but did not use or conversely to be sure you do not get kicked off the sorter by the next user
because you did not book enough time, you should be thoroughly familiar with each of the following four documents.
Just click on the link below to read in a new tab. Click the "about our Core tab" to return.
Noreen Francis | Core Manager
Phone: 617-355-8463
Email: Noreen.Francis@childrens.harvard.edu
The Stem Cell Flow Cytometry Core Facility is a non-profit core laboratory in the Program in Genomics and Genetics Division of Boston Children’s Hospital
offering services for academic research institutions and industry. The majority of Core users are investigators from Boston Children’s Hospital and the
surrounding Harvard affiliates, but the Core also serves laboratories throughout the entire United States. The Core is partially supported by private and federal
funds. Funding is provided in part through The Intellectual and Developmental Disabilities Research Center, The Manton Center for Orphan Disease Research,
The Wellstone Center for Muscular Dystrophy, and The Neuromuscular Disease Project of the Program in Genomics at Boston Children’s Hospital. The overall
objective of the Core is to provide a location where researchers can have access to high quality, low cost cell analysis and sorting services in a timely, affordable manner.
| Hours | Location |
|
Monday - Friday 9:00 AM to 5:30 PM |
Room 15037: Cell Analysis and Sorting Center for Life Sciences Building 3 Blackfan Circle Boston, MA 02115
|
Here is a very useful link to a site that describes the basic concept of flow cytometry:
http://flowcytometry.sysbio.med.harvard.edu/documents/IntroNanoCourse.pdf
There are two other Flow cytometry core facilities at Boston Children's Hospital that are closely associated with the IDDRC Flow cytometry Core.
Here are the links to these two sister facilities:
http://www.flowlab-childrens-harvard.com/
http://www.idi.harvard.edu/about_us/core_facilities/flow_and_imaging_cytometry
This link will help you understand multicolor flow analysis:
http://www.bdbiosciences.com/research/multicolor/tools/index.jsp?WT.ac=Feature_Colortools_B
This link will help you to understand many stem cell concepts:
http://www.biolegend.com/stemcell
Nearby by to us is the excellent Flow cytometry Core at the Dana-Farber Cancer Institute.
In the Resources section of their web site you will find a rich source of resources such as "Spectral Viewers;
Experimental Design and Analysis tools; Societies and Vendors. Here is that link:
http://flowcytometry.dfci.harvard.edu/index.php/resources
Also at the Dana Farber site under the policies section you will find many policy statements
that also apply to the IDDRC Flow Cytometry Core.
Here is Dana-Farber Cancer Institute flow cytometry core where you will find many more links to resources and vendors .
http://flowcytometry.dfci.harvard.edu/index.php/policies
Here is a link where you can find manuals and tutorials for Flowjo software and compensation.
http://crmflow.partners.org/library.php
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Ronald Mathieu |
Ronald.Mathieu@childrens.harvard.edu
|
