ATTENTION ⚠️
On Saturday April 20th, 2024 at 8:00 AM (UTC), emergency site maintenance has been scheduled for iLab Primary U.S. Details and updates can be found on the Agilent Status Page. For additional information, please contact the Agilent iLab Support by clicking on 'Help' at the top of your account page or by emailing ilab-support@agilent.com.
For price inquiry, please email Catherine Brownstein at catherine.brownstein@childrens.harvard.edu.
RNA Expression | miRNA Expression | RNAseq | smRNAseq | |
Affymetrix | ● | ● | ||
CFX Real-Time PCR | ● | ● | ||
Nextgen | ● | ● | ||
Fluidigm | ● | ● | ||
Before you can login and request services, you must register an iLAB account by clicking on the "sign up here" link at the top-right of the opening page of the website and fill in all the required information. Once registration is completed, please login and click on "Request Services" tab.
Shipping address:
Molecular Genetics Core Facility
3 Blackfan Circle
CLS 16030.22
Boston MA 02115
We have a new TapeStation 4200, Bionano Saphyr, Bio-Rad QX200 ddPCR system, Hamilton STAR, and a Nanostring! Log in to iLab to reserve time or request as a service.
Leadership:
Catherine Brownstein | Core Manager
Phone: 617-355-4764
Email: catherine.brownstein@childrens.harvard.edu
Hours | Location |
Monday - Friday 9:00 AM to 5:00 PM |
Room 16030 Center for Life Sciences Building 3 Blackfan Circle Boston, MA 02115
|
Name | Role | Phone | Location | |
---|---|---|---|---|
Catherine Brownstein |
Core Manager
|
617-355-4764
|
catherine.brownstein@childrens.harvard.edu
|
CLS 16030
|
Kristin Cabral |
Senior Technician
|
617-919-3382
|
kcabral@enders.tch.harvard.edu
|
CLS 16030.22
|
Julia Phoon |
Research Assistant I
|
617-919-3380
|
julia.phoon@childrens.harvard.edu
|
CLS 16030.21
|
Nathaniel Edisis |
Research Assistant I
|
617-355-5801
|
nathaniel.edisis@childrens.harvard.edu
|
CLS 16030.21
|
Standard Service List |
► Chromosomal Microarray on Agilent SureScan (1) | |||
Name | Description | Price | |
---|---|---|---|
SurePrint G3 Human CGH Microarray 4x180K |
Customers must provide all reagents and slides, including gaskets, wash buffers, and Human Cot-1. This platform enables to quickly and reliably identify aneuploidies, microdeletions, microduplications, as well as other types of chromosomal aberrations across the genome. ; Agilent’s CGH platform offers real comparative genomic data thanks to the 2-color approach, and unlimited flexibility in terms of format and content. Learn more about our catalog and custom microarrays. Coding and non-coding human sequences represented Probes are annotated against NCBI Build 38 (UCSC GRCh38, Dec. 2013) Probe design and selection have been carefully optimized and validated for maximal sensitivity and specificity Agilent provides validated protocols and reagents to process fresh frozen (SureTag Labeling Kit) and FFPE samples (ULS Labeling Kit.) Arrays and reagents included. |
Internal
$309.00
each
External $325.00 each Core Affiliate $294.00 each Industry $358.00 each |
|
► DNA and RNA extractions (1) | |||
Name | Description | Price | |
Self serve DNA and RNA extraction - Qiagen QIACube |
Self service extractions using the Qiagen QIACube. User provides Qiagen kit. We provide reagent bottles, tips, tubes, and rotor adaptors necessary for the Qiacube. The pricing is per run. A run can accomodate 2-12 samples. Contact us via email for reserving the machine. |
Inquire | |
► Data Management (1) | |||
Name | Description | Price | |
Data transfer support |
Assistance with data movement and transfer. 1 hour minimum. |
Inquire | |
► Droplet Digital PCR on Biorad QX200 (1) | |||
Name | Description | Price | |
ddPCR full service |
Pricing listed is per 8 sample (column based). Please enter per column. Investigator provides assay and restriction enzyme if necessary. ***Please clearly specify how materials need to be stored***
|
Inquire | |
► Gene Expression on Fluidigm (1) | |||
Name | Description | Price | |
Fluidigm Biomark Training |
Required training with our staff for use of the MGCF Fluidigm Biomark machine. Hands on training with your own samples. This service only includes the cost of training. Please separately purchase your desired chip format and reagents for training. Please contact the core for more information. |
Internal
$350.00
each
External $400.00 each Core Affiliate $300.00 each Industry $450.00 each |
|
► Gene Expression on Genechip Microarray (3) | |||
Name | Description | Price | |
GeneChip (Affymetrix )-Hyb-Only |
Customer must provide Genechips, hybridization reagents, and washing reagents. Samples have already been labeled. Please inform the core of the protocol used for ampllification and labeling. |
Inquire | |
GeneChip (Affymetrix) Arrays |
Customer must provide Genechips, amplification and labeling reagents, hybridization reagents, and washing reagents. If clean-up components are not included with the kits the customer must provide those as well.
|
Inquire | |
GeneChip (Affymetrix) Arrays for miRNA |
Customer must provide Genechips and FlashTag Biotin HSR RNA Labeling Kit and hybriziation and washing reagents. |
Inquire | |
► Gene Expression on Nanostring nCounter MAX (2) | |||
Name | Description | Price | |
*Nanostring |
NanoString nCounter® MAX Profiler: A simple, cost effective solution for multiplexing 10 – 800 targets in a single tube.
|
Inquire | |
Nanostring- self service |
Price is per run. Please book time under the Equipoment Reservations tab. |
Internal
$126.00
plate
External $133.00 plate Core Affiliate $120.00 plate Industry $147.00 plate |
|
► Mapping on Bionano Saphyr (1) | |||
Name | Description | Price | |
Bionano Saphyr Data Analysis Only |
Analysis of Bionano Saphyr data. Price is per sample. Deliverable is a research report. Please note that the MGCF is NOT a CLIA facility. |
Inquire | |
► Next Gen Sequencing Services (5) | |||
Name | Description | Price | |
FFPE extraction + sequencing |
Please inquire for details and specifications. |
Inquire | |
FFPE extraction + sequencing for tumor Sample |
please inquire for specifications |
Inquire | |
Illumina Exome (WES) 100x (return FASTQ or BAM and vcf, plus annotations and web interface for gene hunting) |
We have had to raise our prices due to a vendor raising their prices. We are monitoring the situation and will lower our prices again as soon as we can.
|
Inquire | |
Methylation EPIC Microarray |
1) Sample: The client will submit a minimum of 1.4ug of gDNA as the starting material. 2) Microarray: The microarray will be performed using Illumina’s Methylation EPIC array using related instrumentations and softwares. 3) Deliverables: Raw data in spreadsheet as the deliverable for this project. The resulting data will be made available via FTP download. 4) TAT: Turnaround time is approximately 3-4 weeks after sample has passed initial QC. 5) Additional note: This project is for research use only. |
Inquire | |
Psomagen Human Whole Exome Sequencing |
Prices change frequently- please email for updated quote.
|
Inquire | |
► QC and Handling (2) | |||
Name | Description | Price | |
IDT Probe Plate QC |
Price Per Two Plates |
Inquire | |
QC and handling for send-outs |
Molecular Genetics Core does QC and send-out.
Price is per 12 samples. 12 sample minimum charge. |
Inquire | |
► qPCR (1) | |||
Name | Description | Price | |
BioRad CFX384 Touch real-time PCR system- Self Use |
This instrument is self service. Please request under the Equipment Reservations tab.
The CFX384 Touch Real-Time PCR Detection System is a powerful and precise real-time PCR instrument in a 384-well format, for researchers who require both ease of use and rapid, high-throughput data acquisition. The CFX384 Touch System utilizes accurate temperature control and sensitive, 4-color detection optics to deliver reliable and repeatable qPCR results for singleplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to four-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful software, the CFX384 Touch System was designed to advance your qPCR. Key Features and BenefitsWith the CFX384 Touch Real-Time PCR Detection System you can:
|
Inquire |