BCH/PCMM Microscopy Core

Overview of Services

 

The Program in Cellular and Molecular Medicine (PCMM) at Children's Hospital operates a Microscopy Core with advanced instrumentation in Light and Electron Microscopy.  While Electron Microscopy can provide the ultimate in resolution, modern Light Microscopy such as Confocal and Multiphoton has the advantage of imaging biochemical process in real time, shedding light on the vastly complex molecular world of cells and tissues. We operate a dedicated Multiphoton-Intravital Microscopy (MP-IVM) Facility, since the year 2000, on the second floor of the Warren Alpert Building at the Harvard Quad.  We were the very first to provide MP-IVM service to Longwood area scientists since that time, with expertise training relevant to the experiment being pursued.

All of the microscopes at the PCMM Microscopy Core are shared equipment and are available to both internal and outside users. However, PCMM users are given priority.

 

     

 

 

Equipment

 

System 1 -Confocal-

Olympus Fluoview FV1000 point scanning confocal system                                               

Configuration: Inverted, with Olympus IX 81 microscope                                                             

Lasers lines: 405, 457, 488, 515, 559, 635 nms                                                                                        

Detectors (PMTs): 4 fluorescence +1 transmission; DIC and spectral imaging capability                                      

Location: 3 Blackfan Circle, 3rd/Fl., Rm03123

 

System 2 -Multiphoton-

Olympus Fluoview FV1200 multiphoton system

Configuration: Upright, with Olympus BX 61 WI microscope                                                              

Lasers: Spectra Physics MaiTai DSHP, tuning range = 690-1020 nm                                             

Detectors: 4 external (non-descanned)                                                                                                         

Location: 200 Longwood Ave., WAB Rm274A

 

System 3 -Spinning Disk Confocal-

Marianas SDC system, with Yokogawa CSU22 Spinning Disk                                                        

Configuration: inverted, with Ziess Axiovert 200M microscope, full enclosure environmental chamber                                                                                                                                                  

Lasers: 491, 561 and 642nm                                                                                                          

Detectors: EM-CCD for confocal, wide-field CCD for wide-field                                                                      

Location: 200 Longwood Ave., WAB Rm175

 

System 4 -TEM-

Joel JEM1400 Transmission Electron Microscope                                                                                  

Features: 80,100 or 120 KV accelerating voltage with LaB6 cathode; tilting cryostage; 16MP cooled CCD camera                                                                 

Location: 3 Blackfan Circle, 3rd/Fl., Rm03078

 

 

Leadership

 

Harry Leung - Core Manager

Email: harry.leung@childrens.harvard.edu

Phone: 617-713-8299

 

 Getting Started

 

  • BCH Users - Register for a new account using the link in the upper right hand corner, or click here.

 

  • Login with your approved credentials.

 

 

 

Location and hours of operation

Hours Locations

Training or Assisted Use: 9:00am - 5:00pm (Monday - Friday);

Self-Use (for trained users): All Hours (24/7)

200 Longwood Ave, Boston, MA 02115;

3 Blackfan Circle, 3/F Boston, MA 02115

Links and Resources

  1. http://www.idi.harvard.edu/about_us/core_facilities/optical_microscopy/

Contacts

Name Role Phone Email Location
Harry Leung
Core Manager
 
617.713.8299
 
harry.leung@childrens.harvard.edu