Mouse Embryonic Stem Cell (ESC) and Gene Targeting Core

Specific Aim

In the NIDDK Cooperative Hematology Specialized Core Center (CHSCC) at Boston Children's Hospital (BCH),we will support the field of benign hematology research by providing crucial resources to investigators, by bringing together interactive members who will move the field forward and recruit new investigators to the area, by enriching the environment for trainees at all levels (undergraduate, graduate, medical school) and by offering Pilot & Feasibility grants for highly promising innovative projects of junior investigators. The CHSCC builds on experience with an existing NIDDK-funded Center of Excellence in Hematology at BCH. The CHSCC will continue to provide services for hematology investigators, and also integrate its activities with the larger consortium. The goal is to have the BCH CHSCC serve as a national resource for the benefit of benign hematology research.

Overview of Services

This Core’s mission is the support and development of genetically engineered mouse models for hematology research. These models may be aimed at determining the in vivo roles of specific genes in the hematopoietic system or at generating mouse strains that mimic corresponding human disorders. This Core has the infrastructure and expertise to generate mouse models through conventional gene targeting in ES cells and through use of the newer CRISPR/cas9 genome engineering platform. As required for specific projects, This Core employs oocyte or blastocyst microinjection. This Core incorporates new methods, such as CRISPR/cas9, as they become available. In addition to generating new genetic strains, we maintain numerous strains for on-going research and for distribution to the hematology community. These strains include numerous Cre expressing mice lines for conditional gene targeting. Although many of these lines are available from resources such as the JAX laboratories, their shelf-ready status accelerates research and avoids delays in importing strains and expanding colonies. This Core is also active in teaching trainees in other laboratories how to handle ES cells, design targeting constructs, and design guide RNAs for CRISPR/cas9 editing. Moreover, this Core assists investigators in analysis of mouse phenotypes, particularly those in embryos. A particular focus of the Core is support of junior investigators.

Location and hours of operation

Contacts