Experimental Neurophysiology Core

Overview of Services

High throughput preclinical neurophysiology is essential for drug discovery in many disease states. The Experimental Neurophysiology Core (ENC) aims to improve the early phases of drug discovery and development by creating central nervous system (CNS) disease models and recording spontaneous and evoked signals from rodents for physiological characterization, target engagement and drug testing applications.

The ENC at Boston Children’s Hospital can acquire a plurality of physiologic signals through in vivo and in vitro recordings to quantitatively assess seizures, circadian abnormalities and physiologic signatures across the EEG frequency spectrum. Available ENC techniques include in vivo rodent video-EEG via multi-cage wireless telemetry, auditory and motor evoked potentials by auditory stimulation or direct cortical and transcranial magnetic stimulation and in vitro recordings from isolated brain slices. The ENC also provides expertise and end-to-end services for rodent surgeries, dosing compounds, collecting samples for pharmacokinetic analysis, and conducting high-throughput data analysis. The state of the art setup enables a strong set of physiological assays to evaluate novel CNS pharmacotherapies in preclinical disease models. Our platform is available to industrial and academic partners to advance with the aim of expanding and improving the pipeline from preclinical evaluation of therapeutic targets to clinical trials.

• In vivo rodent wireless telemetry video-EEG (using DSI systems)
• Noninvasive rat brain stimulation (using transcranial magnetic stimulation – TMS)
• In vitro extracellular isolated brain slice stimulation and recording by microelectrode array (using MED64 system)

Leadership

Alexander Rotenberg, MD, PhD | Core Director
Phone: 617-919-2763 | CLS 14
Email: alexander.rotenberg@childrens.harvard.edu

Amy Weinberg, MPH | Center Administrator
Phone: 617-919-4023 | CLS 12
Email: amy.weinberg@childrens.harvard.edu

Mustafa Sahin, MD, PhD | Neurologist-in-Chief
Phone: 617-355-8994 | CLS 14
Email: mustafa.sahin@childrens.harvard.edu   

Staff

Henry Lee, PhD | Senior Scientist
Email: hingcheong.lee@childrens.harvard.edu | CLS 14063

Lna Ali, MS | Administrator
Phone: 617-919-2766 | CLS 14040.13
Email: lna.ali@childrens.harvard.edu   

Consultants

Sameer Dhamne, MS            
Email: sameer.dhamne@childrens.harvard.edu

Links & Resources

1. In vivo rodent wireless telemetry video-EEG
2. Noninvasive rat brain stimulation
3. In vitro extracellular isolated brain slice stimulation and recording

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